Stalling the Bam complex, the outer membrane protein folding machinery (360G-Wellcome-207033_Z_17_Z)

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The emergence of bacteria that are resistant to available antibiotics represents a growing global threat requiring new strategies to combat infection. Multidrug-resistance is most serious for Gram-negative bacteria, with few antibiotics likely to be available in the near future. The understanding of the Gram-negative bacterial outer membrane (OM) is critical to developing new antimicrobial agents. Proteins present within this membrane play key roles in microbial pathogenesis Almost all proteins within this membrane utilise the Bam complex for correct folding into the outer membrane, as such it is a key target for the development of novel antimicrobials (Hagan et al. 2015). Unfortunately little is known about the molecular mechanisms of how it functions. It has been shown that one particular class of Bam substrate, the autotransporters, can be stalled in vivo by the simple addition of a large disulphide bonded loop within the passenger domain of these proteins (Renn and Clark 2011). The key goal of this study is to trap a disulphide bonded autotransporter within the Bam complex in vitro and to perform an initial characterisation of it. This will lead to providing fundamental information on how the Bam complex functions and potentially how it can be inhibited.

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Grant Details

Amount Awarded 0
Applicant Surname Johal
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207033/Z/17/Z
Lead Applicant Miss India Johal
Partnership Value 0
Planned Dates: End Date 2017-09-02T00:00:00+00:00
Planned Dates: Start Date 2017-07-03T00:00:00+00:00
Recipient Org: Country United Kingdom
Region West Midlands