Characterization of the role of C12orf65 in quality control of human mitochondrial protein synthesis. (360G-Wellcome-207039_Z_17_Z)

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I intend to determine the role C12orf65, a member of the mitochondrial translation release factor family, plays in mitochondrial quality control. Professors Zofia Chrzanowska-Lightowlers and Bob Lightowlers have recently established a technique for mitoribosomal profiling. They have already produced sets of profiling data for control fibroblasts and those artificially depleted of C12orf65 but want to compare this data to cells from a patient with a pathogenic mutation of C12orf65. My project is to learn how to interpret this data and to generate a new library from a patient cell line. Goal 1 - be trained in how to produce and interpret large datasets generated from isolated RNA fragments protected by the mitoribosomes in patient fibroblasts (weeks 1-6). Goal 2 - initiate bioinformatic studies for the interpretation of large dataset produced from a library of short protected RNA species (week 1-6). Goal 3 - prepare protected RNA fragments for library production (week 3-4). Goal 4 - produce a cDNA library from the isolated RNA fragments to allow HiSeq NextGen sequencing (week 5-6). Although I will not be able to assess data from my library, I will be constantly in contact with the Chrzanowska-Lightowlers laboratory who will inform me of the outcome.

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Grant Details

Amount Awarded 0
Applicant Surname Hollis
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207039/Z/17/Z
Lead Applicant Mr Robin Hollis
Partnership Value 0
Planned Dates: End Date 2017-09-02T00:00:00+00:00
Planned Dates: Start Date 2017-07-03T00:00:00+00:00
Recipient Org: Country United Kingdom
Region North East