Purification of membrane transporters to identify topology and binding sites by mass spectrometry (360G-Wellcome-207091_Z_17_Z)

£0

Understanding how membrane transport proteins interact with their substrates is a pressing challenge in biomedical research. In particular, multidrug pumps, which have a very broad substrate specificity, have continued to defy researchers by stubbornly withholding the secrets of their polyspecificity. In this project, the student (Michaela Kompauerova) will instigate a new avenue of research for our group that in the longer-term would enable us to tackle this problem using mass spectrometry. She will express and purify two bacterial ATP binding cassette transporters that show broad substrate specificity (indeed both Sav1866 and BmrA are multidrug pumps), s proof of principle for this approach. Having purified the transporters by the use of novel styrene maleic acid copolymer extraction she will investigate whether these proteins can be identified by mass spectrometry, enabling us to drive forward a new collaboration with protein labelling and mass spectrometry experts in Life Sciences and Chemistry. The longer-term aim of this collaboration is to develop new strategies for determination of membrane protein:substrate interaction surfaces.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 0
Applicant Surname Kompauerova
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207091/Z/17/Z
Lead Applicant Miss Michaela Kompauerova
Partnership Value 0
Planned Dates: End Date 2017-08-04T00:00:00+00:00
Planned Dates: Start Date 2017-06-05T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East Midlands