Exploiting MALDI Imaging Mass Spectrometry to understand effects of plant extracts in a human model for atopic dermatitis (360G-Wellcome-207114_Z_17_Z)

£0

The first aim of this project is to analyse microarray and MALDI-IMS data obtained from an atopic dermatitis (AD) ex-vivo skin model produced in my supervisor's lab by cytokine treatment. A comparison between altered gene expression shown in microarrays will be compared with altered protein expression shown in the MALDI-IMS data. The cytokine treated ex-vivo skin explants treated with resveratrol will also be compared with published proteomic data for AD and psoriasis, as well as comparing cytokine and resveratrol treated skin explants with published data. Once this has been done three key proteins will be identified for further study. The expression of these key proteins will be confirmed in the cytokine and resveratrol treated skin explants through qRT-PCR and immunocytochemistry. Gene expression of the key proteins will involve RNA extraction and the use of qRT-PCR. Immunostaining of the key proteins of skin extract cryo-sections will also be carried out. Serial section will also be stained for specific keratinocyte proliferation markers. In this project, I will gain the key skills of bioinformatic analysis, techniques in skin explant culture, qRT-PCR, immunocytochemistry and Western blotting. I will also be gaining an understanding of the specific localisation of key proteins involved in AD patients.

Where is this data from?

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Grant Details

Amount Awarded 0
Applicant Surname Smith
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207114/Z/17/Z
Lead Applicant Miss Zoe Smith
Partnership Value 0
Planned Dates: End Date 2017-09-02T00:00:00+00:00
Planned Dates: Start Date 2017-07-03T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England