Automated single-cell microscopy of insulin-to-insulin regulation in C. elegans (360G-Wellcome-207136_Z_17_Z)

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Insulin-like peptides (ILPs) play evolutionarily conserved roles in many physiological processes, including development, ageing, and neural function. Humans and other species possess multiple ILPs, and cross-regulation between ILPs is important for biological function. Here we exploit the stereotyped anatomy of C. elegans to dissect the regulation between two ILPs: ins-3 and ins-6. ins-3 negatively regulates ins-6 expression during the control of larval development. ins-3 is expressed in three identified neurons, while ins-6 is expressed in two identified neurons. Our key goal is to map out the regulatory connections from ins-3 neurons to ins-6 neurons. We will determine in which neurons is ins-6 affected in an ins-3 knock-out mutant using an ins-6::mCherry transcriptional reporter. We will next determine from which neurons ins-3 acts from, by examining ins-6 expression in a set of ins-3 mutant strains where wild-type ins-3 is selectively restored to each of its three neurons. All of the above strains have been made and will be analysed with an automated system for fluorescence microscopy to measure ins-6 reporter expression. This work will provide insights into cross-regulation between ILPs at single-cell level, and show how ILPs mediate communication in the nervous system.

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Grant Details

Amount Awarded 0
Applicant Surname Bramer
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207136/Z/17/Z
Lead Applicant Miss Solange Bramer
Partnership Value 0
Planned Dates: End Date 2017-09-16T00:00:00+00:00
Planned Dates: Start Date 2017-07-17T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London