Immunomodulatory effect of cigarette- and electronic cigarette- exposed respiratory pathogens on macrophages in vitro (360G-Wellcome-207215_Z_17_Z)

It has been established that cigarette smoke and E-cigarette vapour can activate inflammatory response and impair antimicrobial functions of human immune cells. However, the direct effects of cigarette smoke and E-cig vapour on respiratory pathogens is less well known. The aim of this project is to further explore the effect of cigarette smoke extract (CSE) and e-cigarette smoke extract (ECSE) on important human respiratory pathogens and their interaction with the host. The immunomodulatory effects of CSE/ECSE exposed respiratory pathogens have on macrophages in vitro will be determined. Western blot analysis will be used to detect activation of the canonical NF-kappaB pathway and caspase-1 activation whilst ELISA assays will be utilized to measure the expression of the cytokines IL-8, IL-12, TNF-alpha and IL-1beta. In addition, susceptibility of bacteria to macrophage phagocytosis and killing will be measured. The findings of this project will provide valuable information about the direct effect of cigarette smoke and e-cigarette on the lung microbiome and its interaction with host immune cells.

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Grant Details

Amount Awarded 0
Applicant Surname French
Approval Committee Internal Decision Panel
Award Date 2017-04-27T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Vacation Scholarships
Internal ID 207215/Z/17/Z
Lead Applicant Ms Elizabeth French
Partnership Value 0
Planned Dates: End Date 2017-08-13T00:00:00+00:00
Planned Dates: Start Date 2017-06-14T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Northern Ireland