Structural cell biology of transport vesicle and organelle biogenesis (360G-Wellcome-207455_Z_17_Z)

£3,445,099

Integral membrane protein cargo are constantly moved in coated tubular/vesicluar carriers between the cell's organelles and its limiting membrane in order to maintain membrane identity and function. That these transport processes are of fundamental importance is reflected by the fact that ~30% of mammalian proteins are either components of the vesicle/tubule transport machinery or are its cargo. Coated vesicular/tubular carrier formation including cargo selection requires the interplay of a network of peripheral membrane proteins and membrane components including phospholipids, small GTPases, docking proteins and the cargo itself. The coat must also prepare and facilitate the carrier for fusion with its target. AP2, AP3, COPI and retromer/VARP based coats along with their accessory/regulatory factors are vital for producing a fully functional endosomal system. We will use a combination of X-ray crystallography, NMR and the fast developing techniques of single particle cryoEM and cryo electron tomography allied with biochemical/biophysical studies to formulate theories concerning the architecture, assembly routes and control/regulation of the formation of these four key tubular/vesicular transport carriers. Specific function abolishing mutations designed on the basis of these studies will allow us to test and further explore our theories in cells using a wide range of in vivo techniques.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 3445099
Applicant Surname Owen
Approval Committee Science Interview Panel
Award Date 2017-07-11T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Principal Research Fellowship Renewal
Internal ID 207455/Z/17/Z
Lead Applicant Prof David Owen
Partnership Value 3445099
Planned Dates: End Date 2024-12-01T00:00:00+00:00
Planned Dates: Start Date 2017-12-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Sponsor(s) Prof Gillian Griffiths