Structural cell biology of transport vesicle and organelle biogenesis (360G-Wellcome-207455_Z_17_Z)
Integral membrane protein cargo are constantly moved in coated tubular/vesicluar carriers between the cell's organelles and its limiting membrane in order to maintain membrane identity and function. That these transport processes are of fundamental importance is reflected by the fact that ~30% of mammalian proteins are either components of the vesicle/tubule transport machinery or are its cargo. Coated vesicular/tubular carrier formation including cargo selection requires the interplay of a network of peripheral membrane proteins and membrane components including phospholipids, small GTPases, docking proteins and the cargo itself. The coat must also prepare and facilitate the carrier for fusion with its target. AP2, AP3, COPI and retromer/VARP based coats along with their accessory/regulatory factors are vital for producing a fully functional endosomal system. We will use a combination of X-ray crystallography, NMR and the fast developing techniques of single particle cryoEM and cryo electron tomography allied with biochemical/biophysical studies to formulate theories concerning the architecture, assembly routes and control/regulation of the formation of these four key tubular/vesicular transport carriers. Specific function abolishing mutations designed on the basis of these studies will allow us to test and further explore our theories in cells using a wide range of in vivo techniques.
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Grant Details
Amount Awarded | 3445099 |
Applicant Surname | Owen |
Approval Committee | Science Interview Panel |
Award Date | 2017-07-11T00:00:00+00:00 |
Financial Year | 2016/17 |
Grant Programme: Title | Principal Research Fellowship Renewal |
Internal ID | 207455/Z/17/Z |
Lead Applicant | Prof David Owen |
Partnership Value | 3445099 |
Planned Dates: End Date | 2024-12-01T00:00:00+00:00 |
Planned Dates: Start Date | 2017-12-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East of England |
Sponsor(s) | Prof Gillian Griffiths |