A multi-user FACS facility at the University of Leicester (360G-Wellcome-208393_Z_17_Z)
We request funds to purchase a BD FACSAria Fusion cell sorter enclosed within a Class-II microbiological safety cabinet (MSC) to establish a multi user cell sorting facility. The FACSAria Fusion is a highly flexible and advanced flow cell based flow cytometer that can perform multi-parameter four-way sorting. The Aria flow cell-based detection method provides greater fluorescence sensitivity than alternative stream-in air based cell sorters, which is critical for many of the proposed studies. We have requested funds for a four laser (blue/red/violet/yellow-green) 18-parameter instrument to enable high dimension analysis of the cells during sorting. The violet laser will enable use of highly sensitive brilliant violet fluorochromes and the yellow-green laser will allow greater use of multiple fluorescent proteins. The instrument can also perform single cell index sorting into 384-well plates, which will enable our research teams to undertake single cell transcriptomics projects and sort CRISPR/Cas9-mediated gene edited cells. The instrument is fully integrated into the biosafety cabinet enabling work to be undertaken with primary patient samples and hazard group 2 pathogens. Maintenance, assisting new users and staff training are essential for such a complex instrument, we have therefore requested funding for an experienced grade 7 manager for a 4-year period.
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Grant Details
Amount Awarded | 290555 |
Applicant Surname | Cowley |
Approval Committee | Multi-User Equipment and Technology Development Committee |
Award Date | 2017-07-06T00:00:00+00:00 |
Financial Year | 2016/17 |
Grant Programme: Title | Multi-User Equipment Grant |
Internal ID | 208393/Z/17/Z |
Lead Applicant | Prof Shaun Cowley |
Other Applicant(s) | Prof Andrea Cooper, Prof Andrew Fry, Prof Chris Brightling, Prof David Cousins, Prof John Schwabe, Prof Marco Oggioni, Prof Martin Tobin, Prof Martyn Mahaut-Smith, Prof Michael Barer |
Partnership Value | 290555 |
Planned Dates: End Date | 2022-01-01T00:00:00+00:00 |
Planned Dates: Start Date | 2018-01-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | East Midlands |