A multi-user FACS facility at the University of Leicester (360G-Wellcome-208393_Z_17_Z)

£290,555

We request funds to purchase a BD FACSAria Fusion cell sorter enclosed within a Class-II microbiological safety cabinet (MSC) to establish a multi user cell sorting facility. The FACSAria Fusion is a highly flexible and advanced flow cell based flow cytometer that can perform multi-parameter four-way sorting. The Aria flow cell-based detection method provides greater fluorescence sensitivity than alternative stream-in air based cell sorters, which is critical for many of the proposed studies. We have requested funds for a four laser (blue/red/violet/yellow-green) 18-parameter instrument to enable high dimension analysis of the cells during sorting. The violet laser will enable use of highly sensitive brilliant violet fluorochromes and the yellow-green laser will allow greater use of multiple fluorescent proteins. The instrument can also perform single cell index sorting into 384-well plates, which will enable our research teams to undertake single cell transcriptomics projects and sort CRISPR/Cas9-mediated gene edited cells. The instrument is fully integrated into the biosafety cabinet enabling work to be undertaken with primary patient samples and hazard group 2 pathogens. Maintenance, assisting new users and staff training are essential for such a complex instrument, we have therefore requested funding for an experienced grade 7 manager for a 4-year period.

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Grant Details

Amount Awarded 290555
Applicant Surname Cowley
Approval Committee Multi-User Equipment and Technology Development Committee
Award Date 2017-07-06T00:00:00+00:00
Financial Year 2016/17
Grant Programme: Title Multi-User Equipment Grant
Internal ID 208393/Z/17/Z
Lead Applicant Prof Shaun Cowley
Other Applicant(s) Prof Andrea Cooper, Prof Andrew Fry, Prof Chris Brightling, Prof David Cousins, Prof John Schwabe, Prof Marco Oggioni, Prof Martin Tobin, Prof Martyn Mahaut-Smith, Prof Michael Barer
Partnership Value 290555
Planned Dates: End Date 2022-01-01T00:00:00+00:00
Planned Dates: Start Date 2018-01-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East Midlands