Regulation of the cellular ESCRT pathway by human T cell leukemia virus type 1(HTLV-1) (360G-Wellcome-211422_Z_18_Z)

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This research seeks to investigate the effect of human T-cell leukemia virus type 1 (HTLV-1) Basic Leucine Zipper (HBZ) protein on the overall function of the endosomal sorting complex required for transport (ESCRT) multivesicular body (MVB) pathway. HTLV-1 is a retrovirus that establishes persistent infection in humans, which cause pathologies like adult T-cell leukemia (ATL) and HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP)[1,2]. Recently, studies have shown an association of HTLV-1 budding with MVBs whose biogenesis is regulated by the ESCRT[3,4] . The ESCRT machinery is an evolutionarily conserved cytosolic group of protein subcomplexes for scission of membrane necks from the interior. Hence, viruses like HTLV-1 usurp this pathway for their survival[5]. This project hopes to establish the effect of HBZ on the ESCRT MVB pathway. Epidermal Growth Factor Receptor (EGFR): Epidermal Growth Factor (EGF) uptake and degradation is widely used as a model to study MVB functionality. This will be performed using an EGF/EGFR uptake and degradation assay in which the uptake and degradation of EGF-ALEXA 595 by HeLa cells will be monitored using a fluorescence microscope in GFP-HBZ positive cells compared to GFP only cells. This will give a measure of the effect of HBZ on the ESCRT MVB pathway[6].

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Grant Details

Amount Awarded 0
Applicant Surname AGNUS MOORTHIRAJ
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211422/Z/18/Z
Lead Applicant Miss HRIDYA AGNUS MOORTHIRAJ
Partnership Value 0
Planned Dates: End Date 2018-08-03T00:00:00+00:00
Planned Dates: Start Date 2018-06-04T00:00:00+00:00
Recipient Org: Country Ireland
Region Ireland