How Salmonella modifies its main antigen to change immunogenicity and phage sensitivity (360G-Wellcome-211439_Z_18_Z)

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Salmonella enterica serovar Typhimurium (STM) is the leading cause of gastroenteritis worldwide. Its outer membrane contains an abundance of lipopolysaccharide (LPS), of which the O-antigen is the outermost component. Modification of the OAg can contribute to the ability of Salmonella to evade host immunity, and we have shown it alters the absorption of specific bacteriophage. In STM O-antigen acetylation is carried out by GtrCBTP1 and OafA, modifying the rhamnose and abequose moiety respectively. These are inner membrane bound O-antigen acetyltransferases that function on the periplasmic side of the inner membrane to acetylate the O-antigen during its biosynthesis. The modification by OafA confers the O:5 serotype of Salmonella and GtrCBTP1 confers resistance to lysis by BTP1 bacteriophage. In this project the student will identify residues important for function in GtrCBTP1 and OafA, through mutagenic analysis and subsequent functional analysis based on serotype change and phage resistance. These OAg acetyltransferases are widespread in salmonellae, giving broader biological relevance. Understanding the biological process of O-antigen acetylation in Salmonella therefore can inform the larger protein family, and may open new avenues for disease control.

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Grant Details

Amount Awarded 0
Applicant Surname Mannsverk
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211439/Z/18/Z
Lead Applicant Mr Steinar Mannsverk
Partnership Value 0
Planned Dates: End Date 2018-09-08T00:00:00+00:00
Planned Dates: Start Date 2018-07-09T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Yorkshire and the Humber