Cellular plasticity in the avian primitive streak - a differential analysis (360G-Wellcome-211609_Z_18_Z)

£0

During gastrulation, mesoderm cells undergo ingression through the primitive streak followed by migration and further specification. Our aim is to investigate how these closely linked processes become integrated by developmental signalling pathways. We showed previously that BMP2 and Wnt3a converge on Smad1 to alter cardiac progenitor cell (CPC) migration in the early chick gastrula; interestingly, ectopically located cells remained cardiogenic. In contrast, paraxial mesoderm progenitors (PMPs) at the mid-gastrula stage responded to elevated BMP2 by changing fate without changing their migration. We have used RNAseq to uncouple the differential responses of early and late mesoderm progenitors to the same signalling network: BMP2/Wnt3a/Smad1. CPC and PMP cells were exposed to BMP2, Wnt3a, or constitutively-active Smad1. RNAseq analysis identified candidate genes that were up- or downregulated in response to all treatments. We have identified, some developmental regulators alongside more specific signal transducers and several novel genes. The summer studentship will contribute to the ongoing validation of these candidate genes. Following confirmation of differential expression by RT-qPRC, the key goal will be to undertake gene expression analysis using whole mount in situ hybridisation in early gastrula stage embryos. The outcome from these experiments will underpin our future functional in vivo analyses.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 0
Applicant Surname Jones
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211609/Z/18/Z
Lead Applicant Miss Bethany Jones
Partnership Value 0
Planned Dates: End Date 2018-09-01T00:00:00+00:00
Planned Dates: Start Date 2018-07-02T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England