Identification of novel regulators and effectors of E-cadherin (360G-Wellcome-211673_Z_18_Z)
The expression of the tumour suppressor gene, E-cadherin, has been found to be positively correlated with the expression of 31 human genes, which have 14 Drosophila homologues. Six of the Drosophila genes are not known to regulate/be regulated by E-cadherin. The aim of this project is to investigate whether E-cadherin regulates the expression of two top candidate genes, fusilli and grainy head, or conversely, if E-cadherin is regulated by them. In order to determine whether fusilli and grainy head regulate E-cadherin, expression of these genes will be downregulated with RNAi using the GAL4-UAS system in Drosophila, and the changes in E-cadherin amounts and localization will be quantified. Simultaneously, E-cadherin will be acutely overexpressed using GAL4-UAS in combination with the temperature-sensitive GAL4-inhibitor GAL80 in order to test if it has an effect on the expression of fusilli and grainy head. Changes in expression will be determined using RT-qPCR. Altogether, this project aims to reveal novel regulators or effectors of E-cadherin.
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Grant Details
| Amount Awarded |
0
|
| Applicant Surname | McNeill |
| Approval Committee | Internal Decision Panel |
| Award Date | 2018-05-31T00:00:00+00:00 |
| Financial Year | 2017/18 |
| Grant Programme: Title | Vacation Scholarships |
| Internal ID | 211673/Z/18/Z |
| Lead Applicant | Miss Jessica McNeill |
| Partnership Value | 0 |
| Planned Dates: End Date | 2018-08-10T00:00:00+00:00 |
| Planned Dates: Start Date | 2018-06-11T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | Yorkshire and the Humber |