Identifying APC7 phosphorylation sites in mitosis and G1 phase of the cell cycle (360G-Wellcome-211718_Z_18_Z)
The proposed research aims to examine the phosphorylation status of APC/C subunits in mitosis and G1 phase of the cell cycle through the use of retinal pigment epithelial (RPE-1) cells which have a diploid chromosome content and are a good model system for investigating APC/C function. Cells will be synchronised in mitosis through the use of spindle inhibitors, or synchronised in G1, following removal of spindle inhibitors. Cell cycle status will be validated by flow cytometry. Immunoprecipitation with APC/C subunit antibodies will then be performed to isolate the APC/C. APC/C phosphorylation status will be assessed by mass spectrometry. The primary aim of this project is to determine the phosphorylation status of APC7 in G1 and mitosis, as this has not been investigated in great detail before. Once we have identified APC7 phosphorylation sites, we will, through genetic manipulation, generate APC7 mutants that inhibit or mimic phosphorylation and investigate the effects of APC7 phosphorylation on cell cycle progression.
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Grant Details
| Amount Awarded |
0
|
| Applicant Surname | Browne |
| Approval Committee | Internal Decision Panel |
| Award Date | 2018-05-31T00:00:00+00:00 |
| Financial Year | 2017/18 |
| Grant Programme: Title | Vacation Scholarships |
| Internal ID | 211718/Z/18/Z |
| Lead Applicant | Miss Sofia Browne |
| Partnership Value | 0 |
| Planned Dates: End Date | 2018-08-31T00:00:00+00:00 |
| Planned Dates: Start Date | 2018-07-01T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | West Midlands |