Local cAMP regulation of the slow after-hyperpolarization (360G-Wellcome-211795_Z_18_Z)

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The slow afterhyperpolarization (sAHP) occurs following trains of action potentials, and it plays a crucial role in regulating neuronal excitability. It determines the timing of action potential firing, thereby modulating neuronal processes including synaptic plasticity. The sAHP has important implications for disease, since elevated neuronal activity is associated with neurological disorders including epilepsy. Currently, little is know of how the sAHP is regulated at the molecular level. The aim of this research project is to investigate at the molecular level how the second messenger cyclic AMP suppresses the sAHP. This summer project will use a combination of molecular biology and electrophysiology to investigate sAHPs in hippocampal CA1 pyramidal neurons in rat brain slices. Two specific aims will be pursued. First, an anchoring disruptor peptide will be applied to determine whether anchoring of cAMP-dependent protein kinase (PKA) is required for sAHP suppression. Second, we will utilise novel tools for manipulating the phosphorylation state of PKA regulatory subunits to investigate the hypothesis that PKA regulatory subunit phosphorylation supports sAHP suppression by PKA. This approach has the potential to reveal important molecular features of an important physiological process with implications for neurological disorders.

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Grant Details

Amount Awarded 0
Applicant Surname Yuan
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211795/Z/18/Z
Lead Applicant Miss Yuchen Yuan
Partnership Value 0
Planned Dates: End Date 2018-09-24T00:00:00+00:00
Planned Dates: Start Date 2018-07-25T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London