Modelling microglial activation in human neuroinflammatory disease (360G-Wellcome-211797_Z_18_Z)

£0

Abnormal activation of the innate immune system within the brain has been linked with a spectrum of brain pathologies, including dementias and neuroinflammatory diseases. Microglia are brain-resident immune cells capable of responding to external circulating cytokines. Recent evidence from mouse models has shown that type I interferon proteins can activate microglial cells, leading to increased phagocytic activity and increased ingestion of synapses on neighbouring neurons. This enhanced synaptic pruning has been hypothesised to drive aspects of neuroinflammatory disease such as neurolupus, where there is prominent activation of the antiviral type I interferon response. The research will involve cell culture of human microglial cells using an induced pluripotency stem cell (iPSC) system and subsequently examine the effects of type I interferon proteins on their function. I will differentiate iPSC cells to iPSC-derived microglia, and evaluate the phenotypic characteristics of these cells using immunocytochemistry, FACS analysis and functional engulfment assays. I will then evaluate the effect of type I interferon on the phenotype and function of these cells. Key Goal 1. To grow and characterise microglia from human iPSC Key Goal 2. To examine the effect of type I interferon on human microglial cells

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Grant Details

Amount Awarded 0
Applicant Surname Lee
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211797/Z/18/Z
Lead Applicant Mr Liam Lee
Partnership Value 0
Planned Dates: End Date 2018-07-30T00:00:00+00:00
Planned Dates: Start Date 2018-05-31T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Scotland