The use of plasma treated graphene oxide in critical limb schema (360G-Wellcome-211916_Z_18_Z)

The aim of this project will be to determine the angiogenic potential of plasma-coated graphene oxide sheets and their effect on cell viability. The objectives will be to place human umbilical cord endothelial cells(HUVECs) in Matrigel with graphene oxides with varying surface coatings on the graphene oxide. First we will use a plasma reactor to modify the surface properties by increasing the O2 and N2 groups on the surface. HUVECs will then be cultured using EGM-2 complete media before being placed onto Matrigel, along with surface treated graphene oxide. In order to determine the cytotoxic effect of each coating, a Live/Dead assay will be carried out on HUVECs that have been cultured in the presence of plasma-treated graphene oxide sheets after a 48hr culture, using fluorescent detection, while the angiogenic properties of each surface coating will be compared against an uncoated control, with complete media with growth factors being used as a positive control, using ImageJ software which will quantify the number/density and size of the vessels compared to the uncoated control. The ideal result would be a coating that has strong angiogenic properties with minimal cytotoxic properties, which would set the ground work for further in-vitro studies.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 0
Applicant Surname Chan
Approval Committee Internal Decision Panel
Award Date 2018-05-31T00:00:00+00:00
Financial Year 2017/18
Grant Programme: Title Vacation Scholarships
Internal ID 211916/Z/18/Z
Lead Applicant Mr Benjamin Chan
Partnership Value 0
Planned Dates: End Date 2018-08-03T00:00:00+00:00
Planned Dates: Start Date 2018-06-04T00:00:00+00:00
Recipient Org: Country Ireland
Region Ireland