Multi-spectral in vivo optical imaging system (360G-Wellcome-212937_Z_18_Z)

The use of bioluminescence reporting has revolutionized the imaging of small animals. Bioluminescence imaging (BLI) provides a robust technique, enabling researchers to gain essential insights into complex biological systems. BLI is used for the tracking of events in vivo, including the longitudinal monitoring of gene expression, tumour growth, enzyme activity, response to experimental drug therapies, and protein-protein interactions. The IVIS Spectrum is a state-of-the-art preclinical optical imaging system that allows for the sensitive detection of bioluminescence and fluorescence, providing a high-throughput platform for the functional whole-body molecular imaging across all organs in rodents. The equipment is simple to use, and the data obtained straightforward to analyse, making it ideal for multiple and non-expert users. The equipment has a vast range of applications across all biomedical disciplines, including the quantification of regenerative stem-cell and therapeutic immune-cell homing; monitoring bacterial infection; and following the development and treatment of tumours. Multispectral BLI can report on the location of multiple cells type or biological events. In vivo fluorescence imaging is capable of detecting targeted and activatable fluorescent probes and reporters against specific molecular processes, allowing in vivo assessment of angiogenesis, apoptosis and matrix remodelling. Cherenkov imaging of radioactive isotopes provides opportunities for radiotracer development.