Mapping the Genes and Neurons that Regulate Sleep Homeostasis (360G-Wellcome-217150_Z_19_Z)
Although sleep is highly conserved and has a major impact on human health, the genetic and neuronal mechanisms that regulate the timing and amount of sleep remain poorly resolved. From a combination of whole-brain activity mapping, targeted gene editing, and behavioural analysis, we have discovered that neurons in the zebrafish preoptic area that express the neuropeptide Galanin become activated during rebound sleep and that proper sleep homeostasis requires functional Galanin. We will now use RNA sequencing, pharmacology, and mutant analysis to dissect the signals that converge onto Galanin-mediated sleep homeostasis. Second, we will leverage the optical translucency of the zebrafish brain and genetically encoded calcium indicators to image Galanin neurons’ activity in relation to known wake-promoting circuits, such as Hypocretin and Noradrenaline neurons, in vivo. Finally, we will use chemogenetic tools to manipulate the activity of Galanin neurons during and after normal and homeostatic rebound sleep to elucidate the functional role these neurons play in sleep regulation.
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Grant Details
Amount Awarded | 1647968 |
Applicant Surname | Rihel |
Approval Committee | Science Interview Panel |
Award Date | 2019-07-16T00:00:00+00:00 |
Financial Year | 2018/19 |
Grant Programme: Title | Investigator Award in Science |
Internal ID | 217150/Z/19/Z |
Lead Applicant | Dr Jason Rihel |
Partnership Value | 1647968 |
Planned Dates: End Date | 2024-10-01T00:00:00+00:00 |
Planned Dates: Start Date | 2019-10-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Greater London |