Enabling imaging of cells and tissues across scales (360G-Wellcome-218278_Z_19_Z)

£333,750

We seek funding for an array tomography scanning electron microscope (SEM) with ATLAS 5 software together with components to upgrade an associated light microscope, to enable 3D correlative light and electron microscopy (CLEM) of cells and tissues with nm resolution. Array tomography SEM allows for the detection, mapping and automated imaging of serial sections of resin embedded samples and is non-destructive technology. This technology is transformative and will enable high resolution 3D correlation of molecular machineries with cellular structure and specific cell and tissue processes. Using this new technology, our initial consortium of PIs will address the following specific objectives: 1. To elucidate the mechanism of 3D patterning during organogenesis (Pichaud) 2. To characterise the fundamental principle that regulate intracellular trafficking of cargoes and membrane, storage and lysosomal degradation (Brodsky, Futter and Schiavo) 3. To determine the molecular basis for the interaction between the HIV virus and its host cells and tissues (Jolly and Marsh) 4. To elucidate the fundamental mechanisms of nuclear remodelling during passage through mitosis (Baum) 5. To elucidate the connectivity underlying circuit function (Hausser and Wilson) Altogether, our proposed studies will elucidate fundamental mechanisms of cell and tissue morphogenesis, homeostasis, function repair and infection.

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Grant Details

Amount Awarded 333750
Applicant Surname Pichaud
Approval Committee Multi-User Equipment and Technology Development Committee
Award Date 2019-07-04T00:00:00+00:00
Financial Year 2018/19
Grant Programme: Title Multi-User Equipment Grant
Internal ID 218278/Z/19/Z
Lead Applicant Prof Franck Pichaud
Other Applicant(s) Dr Buzz Baum, Dr Jemima Burden, Prof Clare Futter, Prof Clare Jolly, Prof Frances Brodsky, Prof Giampietro Schiavo, Prof Mark Marsh, Prof Michael Hausser, Prof Stephen Wilson
Partnership Value 333750
Planned Dates: End Date 2024-09-01T00:00:00+00:00
Planned Dates: Start Date 2019-09-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Greater London