Characterisation of the Type Three Secretion System Effector NleG (360G-Wellcome-097009_Z_11_Z)
Enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli, which share an infection strategy with the mouse-specific pathogen Citrobacter rodentium, colonise the human intestine is conferred by a Type III secretion system that translocates bacterial 'effector' proteins into enterocytes, wherethey subvert cellular pathways and central organelles. The focus of this project is the NleG effectors, which mimic the structure andfunction of eukaryotic RING finger/U-box ubiquitin E3 ligases. While EHEC contains 9 NleG orthologs, EPEC strain E2348/69 and C. rodentium contain one and three homologs respectively. The targets and role in virulence of the NleGs remain unknown. During Bevin's rotation we found that EHEC NleG targets the nucleus, resulting in diminished staining intensity of histone H3, while NleG7 is found diffused in the cytosol. The goals of this project are to: 1. Determine the intracellular trafficking of the NleGs 2. Identify the NleGs' substrate proteins and the role of the NleGs' in cell signalling 3. Determine the impact of the NleGs on nuclear and cytosolic proteins' ubiquitylation and turnover 4. Determine the role of NleGs during infection using in vivo models
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Grant Details
| Amount Awarded | 156706 |
| Applicant Surname | McGeever |
| Approval Committee | Immunology and Infectious Disease Funding Committee |
| Award Date | 2011-08-31T00:00:00+00:00 |
| Financial Year | 2010/11 |
| Grant Programme: Title | PhD Studentship (Basic) |
| Internal ID | 097009/Z/11/Z |
| Lead Applicant | Ms Bevin McGeever |
| Partnership Value | 156706 |
| Planned Dates: End Date | 2015-08-31T00:00:00+00:00 |
| Planned Dates: Start Date | 2011-09-01T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | Greater London |
| Sponsor(s) | Prof Murray Selkirk |