Testicular function in mice lacking sertoli cell androgen receptors and FSH receptors. (360G-Wellcome-078137_Z_05_A)

Testicular differentiation, development and adult function are critically dependent on the Sertoli cells. These cells line the seminiferous tubules and regulate and support germ cell proliferation and differentiation. While it is known that Sertoli cells and spermatogenesis are regulated by follicle stimulating hormone (FSH) and androgen, the relative contribution of each hormone to Sertoli cell activity has remained largely unknown. The recent introduction of relevant mouse models, particularly the FSH-receptor-null (FSHRKO) mouse and the Sertoli cell specific androgen receptor-null (SCARKO) mouse, have provided the potential to dissect out the hormonal mechanisms which regulate Sertoli cell function. We propose to develop mice lacking androgen-receptors on the Sertoli cell AND FSH-receptors or circulating gonadotrophins. This will allow us to determine the extent of Sertoli cell dependence on hormonal stimulation and will show the overall effects that hormones have on Sertoli cell function. Sertoli cell development, and function will be measured in these animals using morphometric techniques and