FTICR-Mass spectrometric proteomic analysis of transcriptional and post-translational protein isoforms and protein-protein interactions in neurodegenerative, signal transduction and cell cycle biology. (360G-Wellcome-081879_Z_06_Z)

We would like to apply for an equipment grant for a FTICR mass spectrometer which will function as a central resource for UCL research programmes in the Dept. of Molecular Neuroscience, the Institute of Neurology, the Department ofMedicine, The Institute for Structural Molecular Biology and Birbeck College. The participating groups provide unique expertise, very substantial current funding and extensive international recognition of scientific excellence. The goal of this application is to potentiate strong existing projects through theprovision of a top of the range instrument for top down proteomics analysis. The core scientific projects include studies of kinases involved in neurodegenerative diseases, of arginine methylation in nitric oxide pathways, cell cycle and cellular differentiation, of membrane receptor signaling systems, of cross-regulation of core pathways (initially feed back from glycolysis to the nucleus) in cellular regulation, of applications of chemicalbiology to infectious disease and to synthesis of non-natural proteins, and ofprotein-protein interactions in bacterial secretion systems involved in bacterial virulence. Occasional use by other projects is also envisaged.