454 sequencing - dental calculus (360G-Wellcome-092799_Z_10_Z)
Ancient or historic human dental calculus has yet to be explotited as a DNA source for oral bacteria. We attempted to extract oral bacteria from dental caclulus using Anglo Saxon remains for a preliminary study, and utilised two approaches for identifying the bacteria: a) Specific primers designed to identify oral bacteria associated with peridontitis and diet type. b) Universal primers (16S rDNA) to investigate the diversity of oral bacteria within the calculus deposits. Presently, only a) has been performed on the Anglo Saxon remains as further measures are necessary to totally remove the background bacterial contamination in laboratory consumables before b) can be efficiently performed. This process is currently underway.
Where is this data from?
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Grant Details
| Amount Awarded | 17880 |
| Applicant Surname | Dobney |
| Approval Committee | Medical History and Humanities Funding Committee |
| Award Date | 2010-04-13T00:00:00+00:00 |
| Financial Year | 2009/10 |
| Grant Programme: Title | Small grant in H&SS |
| Internal ID | 092799/Z/10/Z |
| Lead Applicant | Prof Keith Dobney |
| Partnership Value | 17880 |
| Planned Dates: End Date | 2010-09-13T00:00:00+00:00 |
| Planned Dates: Start Date | 2010-04-14T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | Scotland |