Characterisation of the pharmacology of leelamine, and isolation and characterisation of related endogenous ligands. (360G-Wellcome-095779_Z_11_Z)
Leelamine, a novel diterpene, exhibits potent cannabinoid-like behavioural effects in mice, however its mechanism of action is currently unknown. We would like to characterise a leelamine receptor or binding site using [3H]leelamine in a radioligand binding assay in CB1 receptor knockout mice. This assay will then be used to screen for endogenous leelamine-like compounds in brain. Brain fractions will be separated using low and medium pressure, and normal phase and reverse phase, column chromat ography. The structure of active constituents will be identified using HPLC-MS and NMR. Any leelamine-like compound(s) will be assessed for its activity in the cannabinoid tetrad tests of hypomotility, antinociception, catalepsy, and hypothermia using mice. Overall, we would like to characterise a potentially new neurotransmitter receptor system in the brain, which may lead to new therapeutically useful targets, and the development of new drugs.
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Grant Details
| Amount Awarded | 328471 |
| Applicant Surname | Devane |
| Approval Committee | Basic Science Interview Committee |
| Award Date | 2011-06-22T00:00:00+00:00 |
| Financial Year | 2010/11 |
| Grant Programme: Title | Career Re-Entry Fellowship |
| Internal ID | 095779/Z/11/Z |
| Lead Applicant | Dr William Devane |
| Partnership Value | 328471 |
| Planned Dates: End Date | 2014-09-30T00:00:00+00:00 |
| Planned Dates: Start Date | 2011-09-01T00:00:00+00:00 |
| Recipient Org: Country | Ireland |
| Region | Ireland |
| Sponsor(s) | Prof L Egan |