Probing the structural rearrangements during 40S ribosomal subunit synthesis in yeast (360G-Wellcome-096997_Z_11_Z)
Ribosome maturation involves hundreds of ribosome assembly factors (AFs) that regulate the time-wise association of ribosomal proteins. Although the RNA is the catalytically active component in ribosomes, the ribosomal proteins play crucial roles in rRNA folding and maintenance of long-range rRNA interactions.Intriguingly, preliminary structure probing data from the lab revealed that AFs required for the formation of the 40S head domain dramatically alter the interactions of ribosomal proteins with rRNA, several of which appeared misplaced in the 40S assembly intermediates. This misplacement presumably has a major impact on the overall structure of the rRNA. We hypothesize that AFs function by preventing certain ribosomal proteins from adopting their final conformation in 40S intermediates until final rRNA processing and folding steps have been completed. The proposed work aims to understand the mechanism and functional importance behind this initial misplacement by addressing (i) how this affects the global structure of the 18S rRNA, (ii) how this is linkedto 18S rRNA maturation and (iii) by dissecting the ribosomal protein-rRNA interactions required for the 18S rRNA maturation. The outcomes should provide substantial novel insights into the function of AFs in ribosome remodelling and rRNA folding.
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Grant Details
Amount Awarded | 147915 |
Applicant Surname | Burlacu |
Approval Committee | Molecules, Genes and Cells Funding Committee |
Award Date | 2011-07-12T00:00:00+00:00 |
Financial Year | 2010/11 |
Grant Programme: Title | PhD Studentship (Basic) |
Internal ID | 096997/Z/11/Z |
Lead Applicant | Ms Elena Burlacu |
Partnership Value | 147915 |
Planned Dates: End Date | 2015-09-30T00:00:00+00:00 |
Planned Dates: Start Date | 2011-10-01T00:00:00+00:00 |
Recipient Org: Country | United Kingdom |
Region | Scotland |
Sponsor(s) | Prof Jean Beggs |