Probing the structural rearrangements during 40S ribosomal subunit synthesis in yeast (360G-Wellcome-096997_Z_11_Z)

£147,915

Ribosome maturation involves hundreds of ribosome assembly factors (AFs) that regulate the time-wise association of ribosomal proteins. Although the RNA is the catalytically active component in ribosomes, the ribosomal proteins play crucial roles in rRNA folding and maintenance of long-range rRNA interactions.Intriguingly, preliminary structure probing data from the lab revealed that AFs required for the formation of the 40S head domain dramatically alter the interactions of ribosomal proteins with rRNA, several of which appeared misplaced in the 40S assembly intermediates. This misplacement presumably has a major impact on the overall structure of the rRNA. We hypothesize that AFs function by preventing certain ribosomal proteins from adopting their final conformation in 40S intermediates until final rRNA processing and folding steps have been completed. The proposed work aims to understand the mechanism and functional importance behind this initial misplacement by addressing (i) how this affects the global structure of the 18S rRNA, (ii) how this is linkedto 18S rRNA maturation and (iii) by dissecting the ribosomal protein-rRNA interactions required for the 18S rRNA maturation. The outcomes should provide substantial novel insights into the function of AFs in ribosome remodelling and rRNA folding.

Where is this data from?

This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.

Grant Details

Amount Awarded 147915
Applicant Surname Burlacu
Approval Committee Molecules, Genes and Cells Funding Committee
Award Date 2011-07-12T00:00:00+00:00
Financial Year 2010/11
Grant Programme: Title PhD Studentship (Basic)
Internal ID 096997/Z/11/Z
Lead Applicant Ms Elena Burlacu
Partnership Value 147915
Planned Dates: End Date 2015-09-30T00:00:00+00:00
Planned Dates: Start Date 2011-10-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Scotland
Sponsor(s) Prof Jean Beggs