Identification of novel gd T-cell ligands cells using a forward genetic screenin haploid human cells. (360G-Wellcome-097033_Z_11_Z)

ãä T cells are a unique subset of T cells which do not require antigen processing and subsequent presentation by major histocompatibility complex (MHC) molecules, but instead recognise antigens directly. However, very little is known about the mechanisms of ãä T-cell ligand recognition. Therefore, we propose a novel gene-trap mutagenesis approach in human haploid cells to identify novel ãä T-cell ligands. There are four primary objectives that need to be met to carry out a successful screen using human ãä T cells and the near-haploid KBM7 cells: (i) establish specific ãä T-cell lines of interest; (ii) prove susceptibility of KBM7 cells to lysis by the particular ãä T-cell subset; (iii) mutagenise the KBM7 cells and