Modulation of the folding energy landscape of a nascent polypeptide by interactions with the ribosome surface. (360G-Wellcome-099767_Z_12_Z)
He we propose identifying and developing a fast-folding protein domain as the new ribosome-nascent chain complex (RNC) system, and using a series of destabilising mutations to determine the effect of ribosome attachement on the foding eneregy landscape. Key goals in this project are the establishment of a suitable RNC system; development of NMR methodology for the study of surface interactions; and ultimately measurement of the effect ofribosomal attachment on the protein folding energy landscape.
£162,047 25 Jun 2012