The role of Bcl3 in skeletal health and disease (360G-Wellcome-099786_Z_12_Z)
In this project we will develop a microfluidic platform to explore both chemical and biochemical co-evolution for the development and understanding ofchemical and biochemical pathways responsible for antibiotic resistance in bacteria. In embarking on this we will develop a multiplexed droplet based evolutionary 'lab on a chip' device that incorporates digital control of chemical and biochemical input. Genetically engineered systems with increased evolvability will be explored with libraries of peptide-antibiotics produced using solvent-resistant micro-peptide droplet synthesis or available librariessynthesized / purchased. The integration of evolution algorithms controlling the micro-device in synergy with the chemical and bacterial libraries will allow us to explore a range of hypotheses for the development of new mechanisms of antibiotic development. For example, can embodied evolutionary approaches be used to accelerate biological directed evolution? Ultimately theaim will be for the chemical-based evolution of small molecules or biologics as a function of computer controlled algorithms allowing us to develop antibiotic drugs in a fundamentally new way.
£150,048 25 Jun 2012