Investigation into the role of ADP-ribosyltransferases in DNA repair. (360G-Wellcome-102348_Z_13_Z)
The focus of this proposed project is to better undestand the role of ADP-ribosyltranferases (ARTs) in DNA repair. PARP1 and PARP2 are known to function in the repair of single strand breaks (SSBs), whereas the recently characterised PARP3 facilitates double strand break (DSB) repair via non-homologous end joining (NHEJ). The specific aims of this project are to determine if any redundancy exists between PARP1 and PARP3 in facilitating DNA repair following DNA single strandbreaks, to determine to the substrates of PARP3 in response to DNA damage, andto determine the contribution of the catalytic domain of DNA damage responsiveARTs to their specific repair outcome. Key goals of the work include generating PARP1 and PARP3 deficient cell lines,both alone and in combination; determining the phenotypes of these disrupted cell lines; optimising mass spectrometry protocols for the reliable detection of in vivo poly ADP-ribosylation; identifying the substrates of PARP3 in response to DNA damage in defined genetic backgrounds; cloning chimeric ARTs
Where is this data from?
This data was originally published by The Wellcome Trust. If you see something about your organisation or the funding it has received on this page that doesn't look right you can submit a grantee amendment request. You can hover over codes from standard codelists to see the user-friendly name provided by 360Giving.
Grant Details
| Amount Awarded | 160309 |
| Applicant Surname | Ronson |
| Approval Committee | PhD Studentships |
| Award Date | 2013-06-24T00:00:00+00:00 |
| Financial Year | 2012/13 |
| Grant Programme: Title | PhD Studentship (Basic) |
| Internal ID | 102348/Z/13/Z |
| Lead Applicant | Mr George Ronson |
| Partnership Value | 160309 |
| Planned Dates: End Date | 2017-09-30T00:00:00+00:00 |
| Planned Dates: Start Date | 2013-10-01T00:00:00+00:00 |
| Recipient Org: Country | United Kingdom |
| Region | South East |
| Sponsor(s) | Prof Kim Nasmyth |