High resolution imaging . (360G-Wellcome-108415_Z_15_Z)

Microscopy is a fundamental tool for studying cellular processes at subcellular resolution, and for dissecting in fine detail the mechanisms by which these occur. Research at the CIMR aims to understand the cellular basis of disease, and the microscopes requested in this application will be vital for our continued study of fundamental cell biological processes, with particular emphasis on membrane trafficking, organelle function, protein homeostasis and folding, the cytoskeleton and autophagy. R esearch goals of the co-applicants highlighted here include the study of adaptor proteins in the formation of specific transport vesicles and control of cargo selection (Robinson lab); the regulation of endosome to Golgi transport (Seaman lab); the roles of myosin motors in membrane transport processes and autophagy (Buss lab); and the mechanisms driving T cell polarization during immune synapse formation and killing (Griffiths lab). The microscopy equipment requested will permit complementary essential analyses for this research, including improved quantification of phenotypes and unbiased cell screening, increased resolution for live cell imaging, improved widefield imaging over longer time periods, and analysis of ultrastructural detail in fixed samples.