Cookies disclaimer

I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

New genetic, imaging and microfluidics technologies for single cell genomics (360G-Wellcome-108438_C_15_Z)

<table> <tbody> <tr> <td>Two major limitations of single cell genomics is (i) the loss of information &nbsp; about the original location within the sample of the sequenced cell and (ii) &nbsp; low throughput at high cost with only hundreds of cells analysed per day. &nbsp; Miniaturising single cell analysis to pico-litre volumes will critically &nbsp; facilitate higher throughput (10^4 - 10^6cells) at lower costs and sidesteps &nbsp; restrictions in handling. Combined with genetic technologies to record &nbsp; lineage history and spatially localise cells this w ill allow questions to be &nbsp; address at single cell resolution that are essential to understand cell &nbsp; diversification following tissue-contextual interactions and the impact it &nbsp; has on gene transcription. 1) We will develop microfluidics based devices to &nbsp; increase sequencing throughput by increasing the number of cells processed at &nbsp; a low cost, and at the same time enabling the complex handling and &nbsp; manipulation of small cell numbers with minimal loss. 2) We will develop &nbsp; genetic technology to: i)&nbsp; record &nbsp; within the genome the lineage history of each cell, for readout at any stage &nbsp; of interest and ii) to provide a unique fluorescent signature to cells to &nbsp; enable us to provide spatial and temporal context to their transcriptional &nbsp; profiles.</td> </tr> </tbody> </table>


11 Jun 2015

Grant details
Amount Awarded 410687
Applicant Surname Hollfelder
Approval Committee Multi-User Equipment Committee (inactive)
Award Date 2015-06-11T00:00:00+00:00
Financial Year 2014/15
Grant Programme: Title Technology Development Grant
Internal ID 108438/C/15/Z
Lead Applicant Dr Florian Hollfelder
Other Applicant(s) Dr John Marioni, Dr Sarah Teichmann, Prof Christopher Ponting, Prof Shankar Srinivas, Prof Georg Holländer, Prof Thierry Voet, Prof Wolf Reik
Planned Dates: End Date 2020-07-31T00:00:00+00:00
Planned Dates: Start Date 2015-08-01T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England
Additional data added by GrantNav