The characterisation of T7 polymerase amplifier devices and arsenic repressor/promoter combinations in B. subtilis for use in the development of an Arsenic Biosensor. (360G-Wellcome-202145_Z_16_Z)

£2,000

Harnessing the natural arsenic sensing ability of the bacteria Bacillus subtilis, it has been possible to engineer strains that synthesise a visible pink chromoprotein at different threshold concentrations of arsenic (10, 50 and 200 ppb), corresponding to WHO and government-recommended guidelines for arsenic levels. The constructed strains now require extensive characterisation of individual components of the system. This project aims to characterise the signal-amplifying devices for T7 polymerase and split T7 polymerase, along with several arsenic repressor-promoter combinations. This will be achieved by transferring the desired devices into a pre-designed dual reporter strain of B. subtilis by PCR/molecular cloning/chromosomal integration. The production of fluorescent reporters in plate reader assays for different arsenic concentrations, will allow characterisation of the device output. The performance of these parts and devices across a range of arsenic concentrations and incubation regimes (temperatures, fluctuating temperatures etc.) will also be addressed. The development of an Arsenic Biosensor would effectively address the global issue of arsenic contamination of ground water in a low cost, accessible and sensitive manor.

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Grant Details

Amount Awarded 2000
Applicant Surname Nawrocka-Weekes
Approval Committee Internal Decision Panel for C&S
Award Date 2016-04-01T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Vacation Scholarships
Internal ID 202145/Z/16/Z
Lead Applicant Ms Magdalena Nawrocka-Weekes
Partnership Value 2000
Planned Dates: End Date 2016-08-12T00:00:00+00:00
Planned Dates: Start Date 2016-06-13T00:00:00+00:00
Recipient Org: Country United Kingdom
Region East of England