Characterisation of the Physical Interaction Between the HTLV-1 HBZ Protein and IRF7 (360G-Wellcome-202393_Z_16_Z)
This research hopes to ascertain the effect of the Human T-Lymphotropic Virus Type 1 (HTLV-1) Basic Leucine Zipper (HBZ) protein on Interferon Regulatory Factor 7 (IRF7). The first objective is to determine the intracellular localisation of HBZ and IRF7 in HeLa cells. To achieve this HeLa cells will be grown on glass slides and transfected with plasmids encoding GFP-HBZ and FLAG-IRF7. IRF7 expression will be detected using an antibody against IRF7 followed by a secondary antibody linked to ALEXA-595 (Red). The localisation of proteins in the cells will be visualised using a fluorescent microscope. The second objective is to map the domains in HBZ that are involved in binding IRF7. To achieve this 293T cells will be transfected with plasmids encoding FLAG-IRF7 together with plasmids encoding HBZ-HIS wildtype or HBZ deletions HBZDAD-HIS, HBZDCD-HIS and HBZDbZIP-HIS. Co-immunoprecipitation assays will then be carried out using FLAG resin and precipitates will be analysed by Western Blot.
£2,000 01 Apr 2016