Molecular viable count testing in Pseudomonas chronic bronchial infection (360G-Wellcome-202457_Z_16_Z)

£2,000

In this project, a novel Molecular Bacterial Load (MBL) assay that is able to quantify viable Pseudomonas in samples will be developed. Primers will specifically target the 16SrRNA in Pseudomonas bacteria. This will allow the assay to determine the presence of Pseudomonas bacteria in samples, as well as quantify the number of organisms that are alive. Compared to current techniques for determining the presence and viability of organisms in acute and chronic Pseudomonas infections, the MBL assay will provide results significantly faster, and will be much more specific. This means that the technology could be applied clinically as a new tool to monitor the response of patient to antimicrobial therapy, and ensure that there is not resistance to the selected treatment. This is relevant particularly in patients with chronic respiratory disease such as Cystic Fibrosis, where a chronic (and inadequately treated) Pseudomonas infection can significantly increase morbidity and risk of mortality. Key objectives for this project include: developing a 16SrRNA primer for Pseudomonas to be used in the MBL assay; to determine the specificity of the assay by testing it against organisms that are taxonomically related to Pseudomonas; and to validate the assay with a pre-existing clinical sample set.

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Grant Details

Amount Awarded 2000
Applicant Surname Karmen
Approval Committee Internal Decision Panel for C&S
Award Date 2016-04-01T00:00:00+00:00
Financial Year 2015/16
Grant Programme: Title Vacation Scholarships
Internal ID 202457/Z/16/Z
Lead Applicant Miss Lindsey Karmen
Partnership Value 2000
Planned Dates: End Date 2016-07-31T00:00:00+00:00
Planned Dates: Start Date 2016-06-06T00:00:00+00:00
Recipient Org: Country United Kingdom
Region Scotland