Molecular mechanisms regulating spinal cord regeneration (360G-Wellcome-205894_Z_17_Z)

The transcriptome of spinal cord injuries (SCI) have been reported from different species such as mouse, rat, zebrafish and Xenopus. These experiments led to a consensus that the immune response has a negative effect on the recovery following SCI and the characterisation of "regenerative-associated genes" (RAGs). However, these experiments have yet to uncover the intrinsic mechanisms promoting spinal cord during regeneration. To uncover the genes and mechanisms upregulated during spinal cord regeneration, we will compare the transcriptome of the spinal cord three days after amputation (start of the spinal cord re-growth) in wild type and foxm1-/- knockout Xenopus tadpoles. Foxm1 is a transcription factor that we have identified as being specifically expressed in the regenerated spinal cord and is required for an efficient regeneration of the spinal cord. This is a novel approach as only a handful of transcriptomic experiments of neuronal regeneration using knock out models have been reported. This project will generate a dataset of new molecular players operating during spinal cord regeneration. This novel dataset will provide us with ample preliminary data for further funding, aimed at exploring the mechanisms of spinal cord regeneration, in particular its promotion in both amphibians and mammals.