Adult-Neurogenesis and Network Remodeling on Zebrafish Telencephalon (360G-Wellcome-210219_Z_18_Z)
Zebrafish exhibits adult neurogenesis throughout their brain, conferring them the amazing ability to regenerate damaged brain regions. The study of network remodeling by adult neurogenesis involves complex relationships between newly-added neurons, preexisting networks, and neuronal activity. This proposal combines my expertise on adult-neurogenesis and information processing, the zebrafish capability to perform network remodeling, and the available genetic tools to label different zebrafish neuronal types, as: progenitors, glutammatergic and GABAergic neurons. By combining these transgenic fishes with BrdU tracing, molecular marker detection and electrophysiology, we will able to perform a rigorous and elegant characterization of the different neuronal repertoire contributed by adult neurogenesis to telencephalic pallial networks. Furthermore, the impact of cognitive function on network remodeling will be tested by in vivo two-photon Ca2+ imaging on different cohorts of new-neurons. This project will be the seed for further research on functional neuronal regeneration and de novo wiring of pallial regions, assessed by the physiological and behavioral recovering of lesioned brain.
£96,037 04 Dec 2017