Imaging Flow Cytometry (360G-Wellcome-212908_Z_18_Z)

We seek funding for an ImageStream X Mark II Imaging Flow Cytometer to be hosted by the University of Nottingham (UoN) Flow Cytometry Facility. This exciting technology combines the speed, statistical robustness and multi-parametric data of conventional flow cytometry with the detailed localisation information of microscopy. The machine has been specified following a trial demonstration and in consultation with our extensive user group and co-applicants. It includes five lasers, high magnification, extended depth of field and automated sampler options that would make it one of the most capable machines in its class in the UK. An imaging cytometer would significantly enhance research capability by offering unique high number and high content experiments. Novel applications include the ability to detect, enumerate and characterise rare cells; the ability to dissect cell signalling events through precise measurements of nuclear translocation events or co-localisation; the study of extracellular vesicles in both eukaryotic and prokaryotic systems; precise quantification of drug delivery/internalisation; and the study of cell death mechanisms. We have established mechanisms for access, charging and an extensive client list that have indicated keen interest in this technology, and have secured a 30% financial contribution from the University demonstrating the strategic importance of this equipment.