A New Durable Read EXtension Method for Very, Very Long Reads (360G-Wellcome-212965_Z_18_Z)

There has been a surge of interest in long read sequencing, driven by recent advances in sequencing technology. Long reads have numerous advantages compared to traditional ‘NGS’ approaches because they are able to generate highly complete de novo genome assemblies. These are critical when creating an initial reference sequence for an organism. Long reads are also vital for resolving complex structural rearrangements within populations, as well as being critical in understanding the development and progression of cancer. However, whilst developments by long read sequencing platform vendors provide the underlying platform chemistry compatible with long read applications, there is a skills and knowledge gap for users wishing to use these methods on their own samples. We therefore propose to establish a technology development programme to serve a broad community of researchers interested in benefitting from these techniques. We will focus on three important areas: DNA extraction: evaluating DNA extraction methodologies for a diverse range of animal, plant and microbial samples for long read sequencing DNA library preparation: new methods and refined methods for generating sequencing libraries from high molecular weight DNA Automation of above steps: development of automation-friendly protocols for generation of ultra-long reads (up to and above 1 megabase)